EXPRESSION OF HLA CLASS-I AND CLASS-II (DR, DP AND DQ) DETERMINANTS IN FETAL AND POSTNATAL SALIVARY-GLANDS

Fetal (n = 20) and postnatal (n = 40) parotid glands were examined by two-colour immunohistochemistry combining monoclonal and polyclonal antibody reagents to study the expression of HLA class I and II (DR, DP, and DQ), CD45 and CD3. The epithelium lacked class II during fetal life, whereas class I determinants appeared in some acini and most major ducts. Fetal vessels were positive for both class I and class II (mainly DR), suggesting constitutive expression. Some class II-positive (DR > DP > DQ) histiocytic cells, scattered CD45+ leucocytes, and very few CD3+ T cells were present in the fetal stroma. The epithelium remained DR-negative the first few weeks after birth, but brisk expression was seen subsequently. DP and DQ remained virtually negative in the epithelium throughout the first year. A slight postnatal increase of class II expression (DR > DP > DQ), along with an apparent decrease in class I, was observed in the endothelium. The number of class II-positive histiocytic cells, CD45+ leucocytes and CD3+ T cells, as well as the proportion of presumably activated (DR+) T cells, increased a few weeks after birth. The local immune system hence seemed to be stimulated by extrinsic factors, but the overall number of T cells nevertheless remained small. Stimuli other than T cell-derived lymphokines, therefore, probably explained the brisk postnatal epithelial DR induction.