Quantification of Starch in Guard Cells of Arabidopsis thaliana

被引：10
|
作者：
Fluetsch, Sabrina [1 ]
Distefano, Luca [1 ]
Santelia, Diana [1 ]
机构：
[1] Univ Zurich, Dept Plant & Microbial Biol, Zurich, Switzerland
来源：
BIO-PROTOCOL | 2018年 / 8卷 / 13期
基金：
瑞士国家科学基金会;
关键词：
Starch; Guard cells; Arabidopsis; Stomatal opening; Propidium iodide;
D O I：
10.21769/BioProtoc.2920
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In this protocol, we describe how to quantify starch in guard cells of Arabidopsis thaliana using the fluorophore propidium iodide and confocal laser scanning microscopy. This simple method enables monitoring, with unprecedented resolution, the dynamics of starch in guard cells.
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收藏
页数：8
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