SYNTAXIN - A SYNAPTIC PROTEIN IMPLICATED IN DOCKING OF SYNAPTIC VESICLES AT PRESYNAPTIC ACTIVE ZONES

被引：1103

作者：

BENNETT, MK ^{[1
]}

CALAKOS, N ^{[1
]}

SCHELLER, RH ^{[1
]}

机构：

[1] STANFORD UNIV, MED CTR, BECKMAN CTR MOLEC & GENET MED, STANFORD, CA 94305 USA

来源：

SCIENCE | 1992年 / 257卷 / 5067期

关键词：

D O I：

10.1126/science.1321498

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Synaptic vesicles store neurotransmitters that are released during calcium-regulated exocytosis. The specificity of neurotransmitter release requires the localization of both synaptic vesicles and calcium channels to the presynaptic active zone. Two 35-kilodalton proteins (p35 or syntaxins) were identified that interact with the synaptic vesicle protein p65 (synaptotagmin). The p35 proteins are expressed only in the nervous system, are 84 percent identical, include carboxyl-terminal membrane anchors, and are concentrated on the plasma membrane at synaptic sites. An antibody to p35 immunoprecipitated solubilized N-type calcium channels. The p35 proteins may function in docking synaptic vesicles near calcium channels at presynaptic active zones.

引用

页码：255 / 259

页数：5

共 43 条

[1] SYNAPTOBREVIN - AN INTEGRAL MEMBRANE-PROTEIN OF 18000 DALTONS PRESENT IN SMALL SYNAPTIC VESICLES OF RAT-BRAIN [J].

BAUMERT, M ;

MAYCOX, PR ;

NAVONE, F ;

DECAMILLI, P ;

JAHN, R .

EMBO JOURNAL, 1989, 8 (02) :379-384

[2]

BENNETT MA, UNPUB

[3] SYNAPTIC VESICLE MEMBRANE-PROTEINS INTERACT TO FORM A MULTIMERIC COMPLEX [J].

BENNETT, MK ;

CALAKOS, N ;

KREINER, T ;

SCHELLER, RH .

JOURNAL OF CELL BIOLOGY, 1992, 116 (03) :761-775

[4]

BORDIER C, 1981, J BIOL CHEM, V256, P1604

[5]

CALAKOS N, UNPUB

[6] RAPID ACTIVATION OF HIPPOCAMPAL CASEIN KINASE-II DURING LONG-TERM POTENTIATION [J].

CHARRIAUTMARLANGUE, C ;

OTANI, S ;

CREUZET, C ;

BENARI, Y ;

LOEB, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (22) :10232-10236

[7] A NOVEL ARACHIDONIC ACID-SELECTIVE CYTOSOLIC PLA2 CONTAINS A CA2+-DEPENDENT TRANSLOCATION DOMAIN WITH HOMOLOGY TO PKC AND GAP [J].

CLARK, JD ;

LIN, LL ;

KRIZ, RW ;

RAMESHA, CS ;

SULTZMAN, LA ;

LIN, AY ;

MILONA, N ;

KNOPF, JL .

CELL, 1991, 65 (06) :1043-1051

[8] EUKARYOTIC PROTEINS EXPRESSED IN ESCHERICHIA-COLI - AN IMPROVED THROMBIN CLEAVAGE AND PURIFICATION PROCEDURE OF FUSION PROTEINS WITH GLUTATHIONE-S-TRANSFERASE [J].

GUAN, KL ;

DIXON, JE .

ANALYTICAL BIOCHEMISTRY, 1991, 192 (02) :262-267

[9]

HEUSER JE, 1977, HDB PHYSL NERVOUS SY, V1, pCH8

[10] RAPID VISUALIZATION OF PROTEIN BANDS IN PREPARATIVE SDS-POLYACRYLAMIDE GELS [J].

HIGGINS, RC ;

DAHMUS, ME .

ANALYTICAL BIOCHEMISTRY, 1979, 93 (02) :257-260

← 1 2 3 4 5 →