Effect of cold aqueous plant extract (Citrus aurantium and Solanum melongena) against the Giardia lamblia parasite and Entamoeba histolytica in vitro and measuring the LD50 for extracts in Al-Muthanna province

The current study was conducted in the laboratory of the postgraduate college of Sciences / University of Muthanna. To investigate the parasite Giardia lamblia parasite and Entamoeba histolytica among the reviewers for each of Rumaitha General Hospital, Children's Hospital and childbirth, and during this period the collection of 100 stool specimens of patients with diarrhea. The samples were visually and microscopically examined using a direct swab, and results showed the presence of the parasite Giardia lamblia by 10.8%. As Entamoeba histolytica increased by 16.66%, and the study included parasite culture Giardia lamblia in the TYIS- 33adapting medium and proven successful, as well as the cultivation of a parasitic Entamoeba histolytica condition in beef liver infusion medium, and after the culture has been experimenting with use of cold aqueous for each Peel of citrus aurantium, peel solanuomme longena with three concentrations(100,75,50%) and it turned out that these extracts are effective in eliminating both parasites, and showed a concentration of 75% and a clear impact in the elimination of both parasites, It also found significant differences between different concentrations under the moral level(P= 0.01) and(P= 0.05). and showed 100% concentration effect by the largest in the elimination of both parasites. The median lethal dose account of plant extracts water aqueous for each of the, bitter orange peel Citrus aurantium, eggplant Solanuomme longena if the dose is lethal to extract bitter orange (7.75) g / kg body weight median lethal dose to extract eggplant (7.25) g / kg body weight.