A STANDARDIZED METHOD FOR THE IN-VIVO MAINTENANCE OF CRYPTOCARYON IRRITANS (CILIOPHORA) USING THE GRAY MULLET CHELON LABROSUS AS AN EXPERIMENTAL HOST

被引：26

作者：

BURGESS, PJ

MATTHEWS, RA

机构：

[1] Department of Biological Sciences, University of Plymouth, Drake Circus

来源：

JOURNAL OF PARASITOLOGY | 1994年 / 80卷 / 02期

关键词：

D O I：

10.2307/3283760

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Experimental primary infections with Cryptocaryon irritans were successfully established in mullet following exposure to theronts and maintained for up to 34 successive cycles in this host. Fish were exposed to measured numbers of theronts collected within 4 hr of excystment. Free-swimming trophonts and reproductive cysts were maintained in wells of tissue culture plates containing sterilized seawater and subjected to a photoperiod of 12 hr light: 12 hr dark. Excystment was found to be asynchronous over a period from 84 hr to 35 days. The procedure provided at least a 10-fold yield of parasite material per cycle of transmission.

引用

页码：288 / 292

页数：5

共 20 条

[1] DEMONSTRATION OF A CELL-FREE FACTOR INVOLVED IN CELL-INTERACTIONS DURING MATING IN TETRAHYMENA [J].