CHARACTERIZATION OF PLASMIDS ISOLATED FROM RHIZOBIUM-MELILOTI

被引：22

作者：

SPITZBARTH, M ^{[1
]}

PUHLER, A ^{[1
]}

HEUMANN, W ^{[1
]}

机构：

[1] UNIV ERLANGEN NURNBERG,LEHRSTUHL MIKROBIOL,INST MIKROBIOL & BIOCHEM,D-8520 ERLANGEN,FED REP GER

来源：

ARCHIVES OF MICROBIOLOGY | 1979年 / 121卷 / 01期

关键词：

Cryptic plasmids; Influence of the RP4 plasmid on effectivity; Rhizobium meliloti; Symbiosis;

D O I：

10.1007/BF00409198

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The occurrence of endogenous plasmid DNA in Rhizobium meliloti is reported. R. meliloti strains carrying RP 4 were used to work out a suitable plasmid isolation method. By sarcosyl lysis RP 4 plasmid DNA as well as cryptic plasmid DNA was isolated. Cryptic plasmid DNA possesses a buoyant density of 1.717 g cm-3 and shows a heterogeneity in contour length. Large plasmid molecules of 130 μm length were detected in the electron microscope. We also found that RP4 is able to restore the nitrogen fixation capacity of an ineffective R. meliloti strain. © 1979 Springer-Verlag.

引用

页码：1 / 7

页数：7

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