QUANTITATIVE GAS CHROMATOGRAPHY OF ADRENAL CORTICAL STEROIDS

被引：19

作者：

KITTINGER, GW

机构：

[1] Departments of Biochemistry Oregon Regional Primate Research Center, Beaverton, OR

[2] University of Oregon Medical School, Portland, OR

来源：

STEROIDS | 1968年 / 11卷 / 01期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1016/S0039-128X(68)80051-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new gas chromatographic method for the simultaneous determination of submicrogram quantities of 17-deoxy- and 17 α-hydroxycorticosteroids is described. Corticosteroid mixtures are treated with periodate in aqueous dioxane to quantitatively convert reactive steroids to etiocholenic acid derivatives. Acidic steroids are removed from a portion of the reaction mixture by extraction with aqueous alkali. The remaining neutral and untreated portions are separately treated with butyric anhydride in pyridine to acylate 3- and 17α-hydroxyl groups. Finally, carboxyl groups in the portion untreated by alkali are esterified with diazomethane. Chromatography of the corticosteroid derivatives on SE-30 and QF-1 gives peak areas that permit calculation of the composition of the original mixture. The validity and precision of the method are supported by data given for analysis of mixtures of known composition. © 1968 Holden-Day, Inc.

引用

页码：47 / +

页数：1

共 11 条

[1] THIN-LAYER CHROMATOGRAPHY OF CORTICOSTEROIDS [J].