TRIPLEX FORMATION AT PHYSIOLOGICAL PH BY OLIGONUCLEOTIDES INCORPORATING 5-ME-DC-(N-4-SPERMINE)

被引：37

作者：

BARAWKAR, DA ^{[1
]}

KUMAR, VA ^{[1
]}

GANESH, KN ^{[1
]}

机构：

[1] NATL CHEM LAB,DIV ORGAN CHEM,POONA 411008,MAHARASHTRA,INDIA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 205卷 / 03期

关键词：

D O I：

10.1006/bbrc.1994.2859

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oligonucleotide (ODN) directed tripler formation has therapeutic importance and depends on Hoogsteen hydrogen bonds between a duplex DNA and a third strand. While T*A:T triplets are formed at neutral pH, C+*G:C are favoured at acidic pH. Herein it is shown that 18-mer ODN containing spermine conjugated to 5-Me-dC at N-4 (1-5), form triplexes with complementary 24-mer duplex 8:9 at neutral pH (7.3, 100 mM NaCl). Under such conditions, control ODN's carrying dC (6) or 5-Me-dC (7) did not show any triple helix formation. Remarkably, the triplexes from spermine-conjugates (1-5) have foremost stability at neutral pH (7.1), unlike the behavior of normal ODN's where optimal stability is at acidic pH (5.5). These results have importance in designing oligonucleotides for antigene applications. (C) 1994 Academic Press, Inc.

引用

页码：1665 / 1670

页数：6

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