The effect of Vdr gene ablation on global gene expression in the mouse placenta

被引：2

作者：

Buckberry, Sam ^{[1
]}

Spronk, Fleur ^{[1
]}

Wilson, Rebecca L. ^{[1
]}

Laurence, Jessica A. ^{[1
]}

Bianco-Miotto, Tina ^{[1
,2
]}

Leemaqz, Shalem ^{[1
]}

O'Leary, Sean ^{[1
]}

Anderson, Paul H. ^{[3
]}

Roberts, Claire T. ^{[1
]}

机构：

[1] Univ Adelaide, Sch Paediat & Reprod Hlth, Robinson Res Inst, Adelaide, SA, Australia

[2] Univ Adelaide, Sch Agr Food & Wine, Adelaide, SA, Australia

[3] Univ S Australia, Div Hlth Sci, Sch Pharm & Med Sci, Adelaide, SA 5001, Australia

来源：

GENOMICS DATA | 2015年 / 6卷

基金：

英国医学研究理事会;

关键词：

Vitamin D; Placenta; Gene expression; VDR;

D O I：

10.1016/j.gdata.2015.08.022

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The effects of vitamin D are mediated through the vitamin D receptor (VDR), a predominantly nuclear receptor, expressed in numerous tissues including the placenta. VDR and the retinoid X receptor (RXR) forma dimer complex which binds to genomic vitamin D responsive elements located primarily in promoter regions and recruit cell-specific transcription factor complexes which regulate the expression of numerous genes. To investigate the role of VDR on regulating placental gene expression, mice heterozygous (+/-) for an ablated Vdr allele (C57Bl6 strain B6.129S4-VDRtm1Mbd/J, Jackson Laboratory) were mated to generate Vdr(+/+), Vdr(+/-) and Vdr(-/-) fetuses and placental samples were collected at day 18.5 of pregnancy. RNA was isolated from placental tissue with global gene expression measured using Affymetrix Mouse Gene 2.1 ST Arrays to assess the effects of VDR on global gene expression in the placenta. All raw array data are deposited in Gene Expression Omnibus (GEO) under accession GSE61583. (C) 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

引用

页码：72 / 73

页数：2

共 2 条

[1]

Kauffmann Audrey, 2009, Bioinformatics, V25, P415, DOI 10.1093/bioinformatics/btn647

[2] Vitamin D Receptor Gene Ablation in the Conceptus Has Limited Effects on Placental Morphology, Function and Pregnancy Outcome [J].

Wilson, Rebecca L. ;

Buckberry, Sam ;

Spronk, Fleur ;

Laurence, Jessica A. ;

Leemaqz, Shalem ;

O'Leary, Sean ;

Bianco-Miotto, Tina ;

Du, Jing ;

Anderson, Paul H. ;

Roberts, Claire T. .

PLOS ONE, 2015, 10 (06)

← 1 →