ENZYMATIC-SYNTHESIS OF MANNOBIOSES AND MANNOTRIOSES BY REVERSE HYDROLYSIS USING ALPHA-MANNOSIDASE FROM ASPERGILLUS-NIGER

被引：29
作者：
AJISAKA, K
MATSUO, I
ISOMURA, M
FUJIMOTO, H
SHIRAKABE, M
OKAWA, M
机构：
[1] Meiji Institute of Health Science, Meiji Milk Products Co, Ltd., Odawara, 250
来源：
CARBOHYDRATE RESEARCH | 1995年 / 270卷 / 02期
关键词：
MANNOBIOSES; MANNOTRIOSES; ALPHA-MANNOSIDASE; ASPERGILLUS NIGER;
D O I：
10.1016/0008-6215(95)00015-L
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Various manno-oligosaccharides including alpha-D-man-(1-->2)-D-man and alpha-D-man-(1-->2)-alpha-D-man-(1-->2)-D-man were formed when a highly concentrated mannose solution was incubated in the presence of alpha-mannosidase from Aspergillus niger. alpha-D-Man-(1-->2)-D-man and alpha-D-man-(1-->2)-alpha-D-man-(1-->2)-D-man were isolated by activated carbon chromatography followed by high performance liquid chromatography using an amino-silica column. In addition to the above oligosaccharides, alpha-D-man-(1-->3)-D-man, alpha-D-man-(1 -->6)-D-man, and alpha-D-man-(1-->2)-alpha-D-man-(1-->6)-D-man were also isolated.
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页码：123 / 130
页数：8
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