CRYOPRESERVATION OF INVITRO SHOOT TIPS OF CHICORY (CICHORIUM-INTYBUS L)

被引：0

作者：

DEMEULEMEESTER, MAC ^{[1
]}

PANIS, BJ ^{[1
]}

DEPROFT, MP ^{[1
]}

机构：

[1] CATHOLIC UNIV LEUVEN,TROP CROP HUSB LAB,B-3001 HEVERLEE,BELGIUM

来源：

CRYO-LETTERS | 1992年 / 13卷 / 03期

关键词：

CICHORIUM; CHICORY; CRYOPRESERVATION; CRYOPROTECTION; SHOOT TIP;

D O I：

暂无

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Shoot tips of about 2 mm in length, isolated from in vitro cultured root fragments of chicory (Cichorium intybus L.) variety Flash, were subjected to several cryopreservation protocols. Cooling rates were varied between 0,1-degrees-C/min and 0,7-degrees-C/min and the influence of DMSO (during preculture and cryoprotection treatments) on the survival was examined. The best result (regrowth of 83%) was obtained after 2 days preculture on medium without DMSO, cryoprotection with 15% DMSO and cooling to -40-degrees-C at a rate of 0,5-degrees-C/min prior to immersion in liquid nitrogen.

引用

页码：165 / 174

页数：10

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