CRYOPRESERVATION OF INVITRO SHOOT TIPS OF CHICORY (CICHORIUM-INTYBUS L)

被引：0
|
作者：
DEMEULEMEESTER, MAC [1 ]
PANIS, BJ [1 ]
DEPROFT, MP [1 ]
机构：
[1] CATHOLIC UNIV LEUVEN,TROP CROP HUSB LAB,B-3001 HEVERLEE,BELGIUM
来源：
CRYO-LETTERS | 1992年 / 13卷 / 03期
关键词：
CICHORIUM; CHICORY; CRYOPRESERVATION; CRYOPROTECTION; SHOOT TIP;
D O I：
暂无
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Shoot tips of about 2 mm in length, isolated from in vitro cultured root fragments of chicory (Cichorium intybus L.) variety Flash, were subjected to several cryopreservation protocols. Cooling rates were varied between 0,1-degrees-C/min and 0,7-degrees-C/min and the influence of DMSO (during preculture and cryoprotection treatments) on the survival was examined. The best result (regrowth of 83%) was obtained after 2 days preculture on medium without DMSO, cryoprotection with 15% DMSO and cooling to -40-degrees-C at a rate of 0,5-degrees-C/min prior to immersion in liquid nitrogen.
引用
收藏
页码：165 / 174
页数：10
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