CHARACTERIZATION OF AN AUTOREGULATED RESPONSE ELEMENT IN THE MOUSE RETINOIC ACID RECEPTOR TYPE-BETA-GENE

被引：503
作者：
SUCOV, HM
MURAKAMI, KK
EVANS, RM
机构：
[1] Gene Expression Laboratory, Howard Hughes Medical Institute, Salk Inst. for Biological Studies, San Diego
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 14期
关键词：
Steroid receptor; Transcription; Vitamin A;
D O I：
10.1073/pnas.87.14.5392
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A sequence that confers transcriptional responsiveness to retinoic acid was identified in the promoter of the mouse retinoic acid receptor (RAR) β gene. This response element consists of a direct repeat of the sequence GTTCAC, separated by five nucleotides. Direct binding of the RAR to this sequence was demonstrated by gel retardation and immuno-precipitation assays. This element conferred retinoic acid responsiveness on heterologous promoters via all three subtypes of RAR yet failed to support transcriptional activation by the thyroid hormone, estrogen, glucocorticoid, or vitamin D receptors. Surprisingly, a high level of retinoic acid-dependent activation was seen in the absence of transfected RAR in 10 of 10 vertebrate cell lines, many functionally characterized previously as lacking endogenous receptor. This demonstrates an unusually high sensitivity of the retinoic acid response element to low levels of receptor and suggests expression of RAR in a wide variety of tissue types.
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页码：5392 / 5396
页数：5
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