Expression of Melanocortin-4 Receptor mRNA in Male Rat Hypothalamus During Chronic Stress

被引：0
作者：
Kheirabad, Maryam Karami [1 ]
Jahromi, Bahia Namavar [2 ]
Tamadon, Amin [3 ]
Ramezani, Amin [4 ,5 ]
Ahmadloo, Somayeh [2 ]
Sarvestani, Fatemeh Sabet [2 ]
Koohi-Hosseinabadi, Omid [6 ]
机构：
[1] Azad Univ, Gachsaran Branch, Dept Basic Sci, Gachsaran, Iran
[2] Shiraz Univ Med Sci, Sch Med, Dept OB GYN, Infertil Res Ctr, Shiraz, Iran
[3] Shiraz Univ Med Sci, Transgen Technol Res Ctr, Shiraz, Iran
[4] Shiraz Univ Med Sci, Sch Adv Med Sci & Technol, Dept Med Biotechnol, Shiraz, Iran
[5] Shiraz Univ Med Sci, Inst Canc Res, Shiraz, Iran
[6] Shiraz Univ Med Sci, Lab Anim Ctr, Shiraz, Iran
来源：
INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE | 2015年 / 4卷 / 03期
关键词：
Chronic stress; melanocortin 4 receptor (MC4R); hypothalamus; rats;
D O I：
暂无
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The effects of chronic stress and glucocorticoids receptor antagonist (RU486) on expression of melanocortin 4 receptor (MC4R) mRNA in arcuate nucleus (ARC) of male rats were evaluated. In this study, adult male Sprague Dawley rats were placed into four groups (n=6/group); stress, RU486, stress/RU486, and control groups. In stress group, the rats were restrained, 1 h/day, for 12 days. In RU486 group, the rats were injected RU486 for 12 days. In stress/RU486 group, the rats were injected RU486 1 h before the stress process for 12 days. Relative expression of MC4R mRNA was determined using real-time PCR. Relative expression of MC4R mRNA in the stress group was higher than that of the control rats (P<0.05). Relative expressions of MC4R mRNA were not different between the stress, RU486 and stress/RU486 groups (P>0.05). Chronic restraint stress causes increase in mRNA expression of MC4R in ARC and blockade of glucocorticoid receptors has no effect on this up-regulation.
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收藏
页码：182 / 187
页数：6
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