THIOL-REACTIVE FLUORESCENT-PROBES FOR PROTEIN LABELING

被引：109

作者：

CORRIE, JET

机构：

[1] National Institute for Medical Research, The Ridgeway, London NW7 1AA, Mill Hill

来源：

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1 | 1994年 / 20期

关键词：

D O I：

10.1039/p19940002975

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Cyclisation of N-alkylmaleamic acids mediated by acetic anhydride in dimethylacetamide in the presence of traces of cobalt naphthenate has been used for efficient assembly of a range of fluorescent maleimide reagents. The fluorescence responses of these reagents to addition of thiol across the maleimide double bond, and to hydrolysis of the maleimide ring, are described.

引用

页码：2975 / 2982

页数：8

共 34 条

[21]

MACHIDA M, 1977, CHEM PHARM BULL, V25, P1289

[22]

MACHIDA M, 1977, CHEM PHARM BULL, V25, P1678

[23]

MACHIDA M, 1977, CHEM PHARM BULL, V25, P2739

[24]

Means GE, 1971, CHEM MODIFICATION PR, P105

[25] PHOSPHATE-BINDING PROTEIN OF ESCHERICHIA-COLI [J].

MEDVECZKY, N ;

ROSENBERG, H .

BIOCHIMICA ET BIOPHYSICA ACTA, 1970, 211 (02) :158-+

[26]

METHA NB, 1960, J ORG CHEM, V25, P1012

[27]

NILSSON JLG, 1971, ACTA PHARM SUEC, V8, P497

[28] STUDIES OF PHTHALAZINE-5,8-QUINONE, A RING CONTRACTION, AND SOME NOVEL AND POTENTIALLY USEFUL FLUORESCENT PHTHALIMIDES [J].

PARRICK, J ;

RAGUNATHAN, R .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1993, (02) :211-216

[29] ALKYLATING DERIVATIVES OF AMINO-ACIDS AND PEPTIDES - SYNTHESIS OF N-MALEOYLAMINO ACIDS, [1-(N-MALEOYLGLYCYL)CYSTEINYL]OXYTOCIN, AND [1-(N-MALEOYL-11-AMINOUNDECANOYL)CYSTEINYL]OXYTOCIN - EFFECTS ON VASOPRESSIN-STIMULATED WATER-LOSS FROM ISOLATED TOAD BLADDER [J].

RICH, DH ;

GESELLCHEN, PD ;

TONG, A ;

CHEUNG, A ;

BUCKNER, CK .

JOURNAL OF MEDICINAL CHEMISTRY, 1975, 18 (10) :1004-1010

[30]

Sassiver M. L., 1982, U. S. Patent, Patent No. 4317774

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