THIOL-REACTIVE FLUORESCENT-PROBES FOR PROTEIN LABELING

被引：109

作者：

CORRIE, JET

机构：

[1] National Institute for Medical Research, The Ridgeway, London NW7 1AA, Mill Hill

来源：

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1 | 1994年 / 20期

关键词：

D O I：

10.1039/p19940002975

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Cyclisation of N-alkylmaleamic acids mediated by acetic anhydride in dimethylacetamide in the presence of traces of cobalt naphthenate has been used for efficient assembly of a range of fluorescent maleimide reagents. The fluorescence responses of these reagents to addition of thiol across the maleimide double bond, and to hydrolysis of the maleimide ring, are described.

引用

收藏

页码：2975 / 2982

页数：8

相关论文

共 34 条

[1]

ATWELL GJ, 1984, SYNTHESIS-STUTTGART, P1032

[2] BENZOPYRYLIUM SALTS .8. SYNTHESIS + PROPERTIES OF 7-DIMETHYLAMINOFLAVYLIUM SALTS [J].

BAIRD, WC ;

SHRINER, PL .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1964, 86 (15) :3142-&

[3] ACYLATION OF DIBASIC COMPOUNDS CONTAINING AMINO AMIDINE AND AMINOGUANIDINE FUNCTIONS [J].

BARKER, PL ;

GENDLER, PL ;

RAPOPORT, H .

JOURNAL OF ORGANIC CHEMISTRY, 1981, 46 (12) :2455-2465

[4] REACTIONS OF AMINES .18. OXIDATIVE REARRANGEMENT OF AMIDES WITH LEAD-TETRAACETATE [J].

BAUMGARTEN, HE ;

SMITH, HL ;

STAKLIS, A .

JOURNAL OF ORGANIC CHEMISTRY, 1975, 40 (24) :3554-3561

[5] DIRECT, REAL-TIME MEASUREMENT OF RAPID INORGANIC-PHOSPHATE RELEASE USING A NOVEL FLUORESCENT-PROBE AND ITS APPLICATION TO ACTOMYOSIN SUBFRAGMENT-1 ATPASE [J].

BRUNE, M ;

HUNTER, JL ;

CORRIE, JET ;

WEBB, MR .

BIOCHEMISTRY, 1994, 33 (27) :8262-8271

[6]

Brune M., COMMUNICATION

[7] SYNTHESIS AND FLUORESCENT PROPERTIES OF A NEW PHOTOSTABLE THIOL REAGENT BACM [J].

CHAURASIA, CS ;

KAUFFMAN, JM .

JOURNAL OF HETEROCYCLIC CHEMISTRY, 1990, 27 (03) :727-733

[8] TISSUE SULFHYDRYL GROUPS [J].

ELLMAN, GL .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1959, 82 (01) :70-77

[9] THE SYNTHESIS OF 5-YLIDENEPYRROL-2(5H)-ONES FROM MALEIMIDES AND FROM PYRROL-2-(5H)-ONES [J].

GILL, GB ;

JAMES, GD ;

OATES, KV ;

PATTENDEN, G .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1993, (21) :2567-2579

[10]

GRAVES GD, 1923, J AM CHEM SOC, V45, P2446

← 1 2 3 4 →