ASSAY RESTRICTION PROFILES OF 3 MONOCLONAL-ANTIBODIES RECOGNIZING THE G3M(U) ALLOTYPE - DEVELOPMENT OF AN ALLOTYPE SPECIFIC ASSAY

被引：11
作者：
NELSON, PN
FLETCHER, SM
MACDONALD, D
GOODALL, DM
JEFFERIS, R
机构：
[1] NATL BLOOD TRANSFUS SERV,BIRMINGHAM B15 2SG,ENGLAND
[2] UNIV BIRMINGHAM,SCH MED,DEPT IMMUNOL,BIRMINGHAM B15 2TJ,W MIDLANDS,ENGLAND
[3] CENT RES ESTAB ALDERMASTON,HOME OFF FORENS SCI SERV,ALDERMASTON RG7 4PN,BERKS,ENGLAND
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 138卷 / 01期
关键词：
MONOCLONAL ANTIBODY; GM ALLOTYPE; ELISA; CAPTURE;
D O I：
10.1016/0022-1759(91)90064-M
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Three monoclonal antibodies raised against a purified human IgG3 paraprotein were found to exhibit a restriction profile for IgG3 / G3m(u) and pan-IgG specificity which was dependent on the assay system. When adapted to an IgG3 subclass capture ELISA, all three McAbs discriminated between paraproteins expressing G3m(u) and antithetical markers G3m(st). One of the antibodies (PNF69C) was selected and conditions were optimised for Gm typing purposes. Using this system G3m(u) could be detected on captured IgG3 derived from human sera. This system may prove useful in the elucidation of Gm allotype profiles.
引用
收藏
页码：57 / 64
页数：8
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