RETENTION OF SOME MONOAMINE-OXIDASE INHIBITORY DRUGS ON A BETA-CYCLODEXTRIN POLYMER-COATED SILICA COLUMN

The retention of seventeen monoamine oxidase inhibitory drugs (mono- and disubstituted derivatives of propargylamine) was determined on a beta-cyclodextrin polymer (beta-CDP)-coated silica column using methanol-0.05 M K2HPO4 (6:4, v/v) as the eluent. The inclusion complex formation between the drugs and a water-soluble beta-cyclodextrin polymer was studied by charge-transfer chromatography carried out on reversed-phase TLC layers. The capacity factors were correlated with the various physicochemical parameters and with the inclusion complex-forming capacity of the monoamine oxidase inhibitory drugs. Calculations indicated that the inclusion complex-forming capacity of the drugs does not influence their retention on a beta-CDP column, that is, the water-soluble and water-insoluble beta-CDPs show different retention characteristics. The specific hydrophilic adsorption surface of the solutes significantly influences the retention in HPLC. The results suggest that the selectivity of the beta-CDP-coated silica support may be different from that of the traditional alkyl-bonded reversed-phase columns.