DETERMINATION OF ASCORBIC AND DEHYDROASCORBIC ACID IN POTATOES (SOLANUM-TUBEROSUM) AND STRAWBERRIES USING ION-EXCLUSION CHROMATOGRAPHY

被引：58

作者：

GRAHAM, WD

ANNETTE, D

机构：

[1] Food and Agricultural Chemistry Research Division, Department of Agriculture for Northern Ireland, Belfast, BT9 5PX, Newforge Lane

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 594卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)80329-S

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method has been developed for determination of ascorbic and dehydroascorbic acid. Samples were extracted with 62.5 mM metaphosphoric acid and ascorbic acid determined using an ion-exclusion column with detection at 245 nm. Dehydroascorbic acid was determined after reduction to ascorbic acid. The use of ion-exclusion chromatography enables ascorbic acid to be completely resolved from co-extracted material in both raw and cooked foods. Completeness of separation was confirmed using a photodiode-array detector.

引用

页码：187 / 194

页数：8

共 14 条

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