Localization of Cells Containing Transforming Growth Factor-alpha Precursor Immunoreactivity in the Basal Ganglia of the Adult Rat Brain

被引：55

作者：

Fallon, James H. ^{[1
]}

Annis, Casey M. ^{[1
]}

Gentry, Larry E. ^{[3
]}

Twardzik, Daniel R. ^{[4
]}

Loughlin, Sandra E. ^{[1
,2
]}

机构：

[1] Univ Calif Irvine, Dept Anat & Neurobiol, Irvine, CA 92727 USA

[2] Univ Calif Irvine, Dept Pharmacol, Irvine, CA 92727 USA

[3] Med Coll Ohio, Dept Biochem, Toledo, OH 43699 USA

[4] Oncogen, Seattle, WA 98121 USA

来源：

GROWTH FACTORS | 1990年 / 2卷 / 02期

关键词：

transforming growth factor-alpha; basal ganglia; brain; glia; immunocytochemistry;

D O I：

10.3109/08977199009071510

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Transforming growth factor-a-like immunoreactivity (TGF-alpha-ir) was visualized in the adult rat forebrain using three antisera directed against carboxyterminal sequences in the TGF-alpha precursor. Using immunoperoxidase and immunofluorescence techniques with all three antisera, TGF-alpha-ir was found to be present in a subpopulation of astrocytes in the forebrain. Striatal and pallidal regions of the basal ganglia were studied in detail. In the striatum, there was an uneven distribution of astrocytes containing TGF-alpha-ir, with the greatest number in the dorsal medial third of the caudate-putamen and the overlying corpus callosum/external capsule. In addition, the region of the caudate-putamen bordering the globus pallidus contained numerous clusters of TGF-alpha-ir astrocytes. The globus pallidus itself contained numerous and more evenly distributed TGF-alpha-ir astrocytes. Other pallidal structures-including the ventral pallidum, entopeduncular nucleus, and substantia nigra pars reticulata-contained moderate numbers of TGF-alpha-ir astrocytes. These results suggest that TGF-alpha precursor is present and, perhaps, synthesized in astrocytes. A related growth factor, epidermal growth factor (EGF), has also been reported to be present in pallidal regions of rat brain. Therefore, the TGF-alpha/EGF family of trophic factors may play an important role in the function of the central nervous system.

引用

页码：241 / 250

页数：10