USE OF YARROWIA-LIPOLYTICA HEXOKINASE FOR THE QUANTITATIVE-DETERMINATION OF TREHALOSE 6-PHOSPHATE

被引：1

作者：

BLAZQUEZ, MA ^{[1
]}

GANCEDO, JM ^{[1
]}

GANCEDO, C ^{[1
]}

机构：

[1] CSIC,INST INVEST BIOMED,E-28029 MADRID,SPAIN

来源：

FEMS MICROBIOLOGY LETTERS | 1994年 / 121卷 / 02期

关键词：

TREHALOSE; 6-PHOSPHATE; YARROWIA LIPOLYTICA; HEXOKINASE; YEAST;

D O I：

10.1016/0378-1097(94)90130-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This paper describes a procedure for the quantitative determination of trehalose 6-phosphate (T6P) based on its ability to inhibit hexokinase from Yarrowia lipolytica. The assay is linear between 1 nmol and at least 8 nmol. The concentration of T6P in wild-type Saccharomyces cerevisiae (0.15 mM) and in ras2 mutants (0.25 mM) remained unchanged in the exponential or stationary phase of growth or after heat shock. A tps1 mutant affected in T6P synthase did not show detectable T6P. Heat shock increased the concentration of T6P in Schizosaccharomyces pombe from 0.43 to 0.75 mM.

引用

页码：223 / 227

页数：5

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