USE OF YARROWIA-LIPOLYTICA HEXOKINASE FOR THE QUANTITATIVE-DETERMINATION OF TREHALOSE 6-PHOSPHATE

被引：1
|
作者：
BLAZQUEZ, MA [1 ]
GANCEDO, JM [1 ]
GANCEDO, C [1 ]
机构：
[1] CSIC,INST INVEST BIOMED,E-28029 MADRID,SPAIN
来源：
FEMS MICROBIOLOGY LETTERS | 1994年 / 121卷 / 02期
关键词：
TREHALOSE; 6-PHOSPHATE; YARROWIA LIPOLYTICA; HEXOKINASE; YEAST;
D O I：
10.1016/0378-1097(94)90130-9
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
This paper describes a procedure for the quantitative determination of trehalose 6-phosphate (T6P) based on its ability to inhibit hexokinase from Yarrowia lipolytica. The assay is linear between 1 nmol and at least 8 nmol. The concentration of T6P in wild-type Saccharomyces cerevisiae (0.15 mM) and in ras2 mutants (0.25 mM) remained unchanged in the exponential or stationary phase of growth or after heat shock. A tps1 mutant affected in T6P synthase did not show detectable T6P. Heat shock increased the concentration of T6P in Schizosaccharomyces pombe from 0.43 to 0.75 mM.
引用
收藏
页码：223 / 227
页数：5
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