DETECTION OF 3 PLANT-VIRUSES BY DOT-BLOT AND TISSUE-BLOT IMMUNOASSAYS USING CHEMILUMINESCENT AND CHROMOGENIC SUBSTRATES

被引：17

作者：

MAKKOUK, KM ^{[1
]}

HSU, HT ^{[1
]}

KUMARI, SG ^{[1
]}

机构：

[1] USDA ARS,BARC,FLORIST & NURSERY CORP LAB,BELTSVILLE,MD

来源：

JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT | 1993年 / 139卷 / 02期

关键词：

D O I：

10.1111/j.1439-0434.1993.tb01405.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Detection of bean yellow mosaic virus (BYMV), impatiens necrotic spot virus (INSV) and lily symptomless virus (LSV) by dot-blot and tissue-blot immunoenzymatic assay was compared by using either chromogenic or chemiluminescent substrates. Testing was conducted on infected-leaf extracts and on purified virus preparations. Both types of substrates gave comparable results with leaf extracts. However, with purified virus, the use of chemiluminescent substrates led to a more sensitive detection of the three viruses.

引用

页码：97 / 102

页数：6

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