Phylogenetic analysis of glycoprotein B gene sequences of bovine herpesvirus 1 isolates from India reveals the predominance of subtype 1.1.

被引：11

作者：

Patil, S. S. ^{[1
]}

Prajapati, A. ^{[1
]}

Hemadri, D. ^{[1
]}

Suresh, K. P. ^{[1
]}

Desai, G. S. ^{[1
]}

Reddy, G. B. Manjunatha ^{[1
]}

Chandranaik, B. M. ^{[2
]}

Ranganatha, S. ^{[2
]}

Rahman, H. ^{[3
]}

机构：

[1] Natl Inst Vet Epidemiol & Dis Informat, ICAR, Bengaluru 560064, Karnataka, India

[2] Inst Anim Hlth & Vet Biol, Bengaluru 560024, Karnataka, India

[3] Indian Council Agr Res, Div Anim Sci, Krishi Bhawan, New Delhi 110001, India

来源：

VETERINARY WORLD | 2016年 / 9卷 / 12期

关键词：

bovine herpesvirus 1-1; glycoprotein B; India; infectious bovine rhinotracheitis; phylogenetic analysis; sequencing; subtype; 1.1;

D O I：

10.14202/vetworld.2016.1364-1369

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Aim: This study was conducted for the isolation and molecular characterization of bovine herpesvirus 1 (BoHV-1) isolated from the nasal and vaginal swabs collected from naturally infected cattle showing clinical symptoms of the respiratory disease. Materials and Methods: Isolation of BoHV-1 virus performed on clinical samples collected from 65 cattle from five states of India. The BoHV-1 isolates were further confirmed by polymerase chain reaction (PCR) using primers specific for glycoprotein B (gB) genomic region. PCR amplification was performed using previously published gB gene-specific primer pairs. gB PCR amplicons obtained from all isolates were sequenced, and phylogenetic analysis was performed using software. Results: A total of 12 samples were found positive in cell culture isolation. 11 isolates showed the visible cytopathic effect on Madin-Darby bovine kidney after 72 h. Partial sequence analysis of gB gene of all isolates revealed 99.0-100% homology between them. All isolates showed 99.2-99.8% homology with Cooper stain. Conclusion: BoHV-1.1 is the predominant circulating subtype of BoHV in India, and all isolates have homology with Cooper stain.

引用

页码：1364 / 1369

页数：6

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