EXPRESSION, PURIFICATION, AND CRYSTALLIZATION OF RESTRICTION-ENDONUCLEASE PVUII WITH DNA CONTAINING ITS RECOGNITION SITE

被引：17

作者：

BALENDIRAN, K

BONVENTRE, J

KNOTT, R

JACK, W

BENNER, J

SCHILDKRAUT, I

ANDERSON, JE

机构：

[1] COLD SPRING HARBOR LAB,WM KECK STRUCT BIOL LAB,COLD SPRING HARBOR,NY 11724

[2] NEW ENGLAND BIOLABS INC,BEVERLY,MA 01915

来源：

PROTEINS-STRUCTURE FUNCTION AND GENETICS | 1994年 / 19卷 / 01期

关键词：

ENDONUCLEASE OVEREXPRESSION; CRYSTALLIZATION; X-RAY DIFFRACTION; PROTEIN-DNA COMPLEX; TYPE II RESTRICTION ENZYME; VAPOR DIFFUSION;

D O I：

10.1002/prot.340190110

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have overexpressed the type II restriction endonuclease PvuII (R-PvuII) in E. coli, prepared large amounts of the homogeneous enzyme, and crystallized it with an oligonucleotide carrying a PvuII recognition site. The cocrystals are orthorhombic space group P2(1)2(1)2(1) with cell constants a = 95.8 Angstrom, b = 86.3 Angstrom, c = 48.5 Angstrom, and diffract X-rays to at least 2.7 hi. There is a complex of two protein subunits and one oligonucleotide duplex in the asymmetric unit. (C) 1994 Wiley-Liss, Inc.

引用

页码：77 / 79

页数：3

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