ISOLATION AND CHARACTERIZATION OF MORPHOLOGICAL VARIANTS OF ASPERGILLUS-PARASITICUS DEFICIENT IN SECONDARY METABOLITE PRODUCTION

被引:29
作者
KALE, SP
BHATNAGAR, D
BENNETT, JW
机构
[1] USDA,SO REG CTR,NEW ORLEANS,LA 70179
[2] TULANE UNIV,DEPT CELL & MOLEC BIOL,NEW ORLEANS,LA 70118
来源
MYCOLOGICAL RESEARCH | 1994年 / 98卷
关键词
D O I
10.1016/S0953-7562(09)80411-5
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Polyketide-producing Aspergillus parasiticus was developed as a model system to study fungal strain degeneration. One wild type and five spore colour and auxotrophic mutants of A. parasiticus (designated sec+ for secondary metabolism plus) making aflatoxin and/or pigmented pathway intermediates were subjected to a protocol of serial mycelial transfers in a defined medium. Variant forms (designated sec- for secondary metabolism minus) were isolated from the sec+ forms after 5-12 transfers. The sec- forms exhibited altered morphology, reduced sporulation and inability to make detectable levels of polyketide secondary metabolites. The variants were stable and did not revert to the parental characteristics after more than ten transfers. This pleiotrophic class of non-aflatoxigenic variants serves as a model system to study the commonly occurring, but poorly understood, phenomenon of strain degeneration in filamentous fungi.
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页码:645 / 652
页数:8
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