TRANSFORMING GROWTH-FACTOR-BETA UP-REGULATES HUMAN ELASTIN PROMOTER ACTIVITY IN TRANSGENIC MICE

被引：25

作者：

KATCHMAN, SD ^{[1
]}

HSUWONG, S ^{[1
]}

LEDO, I ^{[1
]}

WU, M ^{[1
]}

UITTO, J ^{[1
]}

机构：

[1] THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,JEFFERSON INST MOLEC MED,DEPT BIOCHEM & MOLEC BIOL,PHILADELPHIA,PA 19107

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 203卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.2208

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have recently developed transgenic mice which express similar to 5.2 kb of the human elastin promoter linked to the chloramphenicol acetyl transferase (CAT) reporter gene (J. Biol. Chem. 269:18072-18075, 1994). Previously, transforming growth factor-beta (TGF-beta) has been shown to enhance elastin gene expression, as determined at the mRNA and protein levels. To examine whether this enhancement could be explained by upregulation of the elastin promoter, TGF-beta 1 (100 ng) was injected subcutaneously into the transgenic animals. CAT activity in the skin of treated animals was elevated in a time-dependent manner up to similar to 10-fold after a single injection. These results suggest that the 5.2-kb up-stream segment of the human elastin gene contains cis-elements responsive to TGF-beta 1 in vivo. (C) 1994 Academic Press, Inc.

引用

页码：485 / 490

页数：6

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