PCR CLONING AND SEQUENCE OF 2 CDNAS ENCODING THE ALPHA-SUBUNIT AND BETA-SUBUNIT OF RABBIT CASEIN KINASE-II

被引：6
|
作者：
GUPTA, SK [1 ]
SINGH, JP [1 ]
机构：
[1] ELI LILLY & CO,CARDIOVASC RES MC 304 D-C 0821,LILLY CORP CTR,INDIANAPOLIS,IN 46285
来源：
GENE | 1993年 / 124卷 / 02期
关键词：
SERINE THREONINE KINASE; SIGNAL TRANSDUCTION; VASCULAR SMOOTH MUSCLE CELL; VASCULAR DISEASE;
D O I：
10.1016/0378-1119(93)90407-T
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Polymerase chain reaction (PCR) amplification of total RNA from vascular smooth muscle cells (VSMC) was used to clone and sequence full-length cDNAs encoding the alpha and beta subunits of rabbit casein kinase-II (CK-II). A strong homology and evolutionary conservation was found in both the nucleotide (nt) and deduced amino acid sequences of CK-II from various species, with up to 100% identity among vertebrate homologues and 88% and 64% identity on the nt level with Drosophila melanogaster and yeast, respectively, compared with rabbit CK-II. The cloning and expression of rabbit CK-II will allow us to generate antibody and cDNA probes to investigate the role of CK-II in VSMC growth, migration, and phenotypic transformation during the pathogenesis of vascular disease.
引用
收藏
页码：287 / 290
页数：4
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