CLONING OF ORGANIC-SOLVENT TOLERANCE GENE OSTA THAT DETERMINES N-HEXANE TOLERANCE LEVEL IN ESCHERICHIA-COLI

被引:52
作者
AONO, R
NEGISHI, T
NAKAJIMA, H
机构
关键词
D O I
10.1128/AEM.60.12.4624-4626.1994
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A variety of genes are involved in determining the level of organic solvent tolerance of Escherichia coli K-12. Gene ostA is one of the genes contributing to the level of organic solvent tolerance. This gene was cloned from an n-hexane-tolerant strain of E. coli, JA300. A JA300-based n-hexane-sensitive strain, OST4251, was converted to the n-hexane-tolerant phenotype by transformation with DNA containing the ostA gene derived from JA300. Thus, the cloned ostA gene complemented the n-hexane-sensitive phenotype of OST4251.
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页码:4624 / 4626
页数:3
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