PRODUCTION OF FUNCTIONAL-RAT HMG1 PROTEIN IN ESCHERICHIA-COLI

被引：41

作者：

BIANCHI, ME

机构：

[1] Dipartimento di Genetica

来源：

GENE | 1991年 / 104卷 / 02期

关键词：

HIGH-MOBILITY GROUP-1 PROTEIN; RECOMBINANT DNA; PROTEIN DNA INTERACTIONS;

D O I：

10.1016/0378-1119(91)90261-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

High-mobility group-1 protein (HMG1) was produced in Escherichia coli under the control of the T7 promoter/T7 RNA polymerase system. The protein can be produced and purified with yields similar to those obtained from animal tissues. HMG1 purified from E. coli is homogeneous and capable of selectively binding cruciform DNA, indicating that post-translational processing of vertebrate HMG1 is not necessary for its DNA-binding ability.

引用

收藏

页码：271 / 275

页数：5

相关论文

共 10 条

[1] INTERACTION OF A PROTEIN FROM RAT-LIVER NUCLEI WITH CRUCIFORM DNA [J].

BIANCHI, ME .

EMBO JOURNAL, 1988, 7 (03) :843-849

[2] SPECIFIC RECOGNITION OF CRUCIFORM DNA BY NUCLEAR-PROTEIN HMG1 [J].

BIANCHI, ME ;

BELTRAME, M ;

PAONESSA, G .

SCIENCE, 1989, 243 (4894) :1056-1059

[3] THE INTRACELLULAR-DISTRIBUTION AND FUNCTION OF THE HIGH MOBILITY GROUP CHROMOSOMAL-PROTEINS [J].

EINCK, L ;

BUSTIN, M .

EXPERIMENTAL CELL RESEARCH, 1985, 156 (02) :295-310

[4] FULL LENGTH CDNA SEQUENCE FOR BOVINE HIGH MOBILITY GROUP-1 (HMG1) PROTEIN [J].

KAPLAN, DJ ;

DUNCAN, CH .

NUCLEIC ACIDS RESEARCH, 1988, 16 (21) :10375-10375

[5] ISOLATION OF HIGH-MOBILITY-GROUP PROTEINS HMG1 AND HMG2 IN NON-DENATURING CONDITIONS AND COMPARISON OF THEIR PROPERTIES WITH THOSE OF ACID-EXTRACTED PROTEINS [J].

MAREKOV, LN ;

DEMIROV, DG ;

BELTCHEV, BG .

BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 789 (01) :63-68

[6] NUCLEOTIDE-SEQUENCE OF RAT-LIVER HMG1 CDNA [J].

PAONESSA, G ;

FRANK, R ;

CORTESE, R .

NUCLEIC ACIDS RESEARCH, 1987, 15 (21) :9077-9077

[7] CARBOHYDRATE MODIFICATIONS OF THE HIGH MOBILITY GROUP PROTEINS [J].

REEVES, R ;

CHANG, D ;

CHUNG, SC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (11) :6704-6708

[8] USE OF BACTERIOPHAGE-T7 RNA-POLYMERASE TO DIRECT SELECTIVE HIGH-LEVEL EXPRESSION OF CLONED GENES [J].

STUDIER, FW ;

MOFFATT, BA .

JOURNAL OF MOLECULAR BIOLOGY, 1986, 189 (01) :113-130

[9] A BACTERIOPHAGE-T7 RNA-POLYMERASE PROMOTER SYSTEM FOR CONTROLLED EXCLUSIVE EXPRESSION OF SPECIFIC GENES [J].

TABOR, S ;

RICHARDSON, CC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (04) :1074-1078

[10] A HUMAN PLACENTAL CDNA CLONE THAT ENCODES NONHISTONE CHROMOSOMAL PROTEIN HMG-1 [J].

WEN, L ;

HUANG, JK ;

JOHNSON, BH ;

REECK, GR .

NUCLEIC ACIDS RESEARCH, 1989, 17 (03) :1197-1214