NEURAL TRANSPLANTATION USING TEMPERATURE-SENSITIVE IMMORTALIZED NEURAL CELLS - A PRELIMINARY-REPORT

Current approaches to neural transplantation–adrenal medullary and fetal brain–suffer from limitations in tissue availability, cellular uniformity, and general applicability. The system described here uses temperature‐sensitive immortalized clonal neural cells to address these limitations. Cerebellar cells, immortalized with a temperature‐sensitive tumor virus, are transformed at 34°C, but differentiate at 38°C. The cells were genetically engineered to express beta‐galactosidase, providing a tag to study their fate in the host. The cells were then successfully transplanted to the cerebellum of adult rats. Copyright © 1990 American Neurological Association