COMPARATIVE BIOCHEMICAL AND IMMUNOLOGICAL STUDIES ON GAMMA-GLUTAMYLTRANSFERASES FROM HUMAN KIDNEY AND RENAL-CELL CARCINOMA APPLYING MONOCLONAL-ANTIBODIES

被引：23

作者：

FISCHER, P ^{[1
]}

SCHERBERICH, JE ^{[1
]}

SCHOEPPE, W ^{[1
]}

机构：

[1] UNIV FRANKFURT KLINIKUM, CTR INTERNAL MED, DEPT NEPHROL, THEODOR STERN KAI 7, W-6000 FRANKFURT 70, GERMANY

来源：

CLINICA CHIMICA ACTA | 1990年 / 191卷 / 03期

关键词：

gamma-Glutamyltransferase (EC 2.3.2.2); Human kidney; Isoenzyme; Monoclonal antibody; Renal cell carcinoma;

D O I：

10.1016/0009-8981(90)90020-S

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

We have purified gamma-glutamyltransferases (GGT) from human kidneys and renal cell carcinomas, and fractionated them according to different lectin-binding properties of the isoenzymes. Native polyacrylamide gel electrophoresis and isoelectric focusing revealed different GGT-bands (even after desialylation) not only among kidney and renal carcinoma, but also among Con A-affine tumor fractions separated by ion-exchange chromatography. Mr of native GGTs were between 106 to 161 kDa, the pI ranged from pH 3 to 4 (pH 5 to 6 after desialylation). Monoclonal antibodies to GGT were produced. One of these, of IgG1 class and designed 138H11, recognizes human kidney GGT and, in addition, GGT from renal cell carcinomas and liver carcinomas. The specificity of mAb 138H11 for GGT was confirmed by Western blotting, by immunohistochemistry and by immunoprecipitation. The potential usefulness of mAb 138H11 in monitoring renal cancer patients and in identification of renal cancer metastases is currently being studied. © 1990.

引用

页码：185 / 200

页数：16

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