DEVELOPMENT OF BIOSENSORS FOR THE DETECTION OF MERCURY AND COPPER IONS

被引：14
|
作者：
HOLMES, DS
DUBEY, SK
GANGOLLI, S
机构：
[1] Department of Biology, Faculty of Sciences, University of Chile, Santiago
来源：
ENVIRONMENTAL GEOCHEMISTRY AND HEALTH | 1994年 / 16卷 / 3-4期
关键词：
D O I：
10.1007/BF01747919
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The development of genetically engineered biosensors for copper and mercury ions is described. The biosensors have been constructed by fusing the lux or light emitting genes from Vibrio fischeri with genetic regulating elements that respond to copper ions or mercury ions, derived respectively from Escherichia coli and Serratia marcescens. The fusions were placed into E. coli cells which then emitted light in response to copper or mercury ions. Data is presented describing the sensitivity, specificity, and dynamic range of the biosensors to their respective target metal ions. A preliminary description of experiments is provided indicating how these biosensors might be used to investigate the bioavailability of mercury and copper ions in environmental samples.
引用
收藏
页码：229 / 233
页数：5
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