ACCELERATED EVOLUTION OF TRIMERESURUS-FLAVOVIRIDIS VENOM GLAND PHOSPHOLIPASE-A2 ISOZYMES

被引:169
作者
NAKASHIMA, K
OGAWA, T
ODA, N
HATTORI, M
SAKAKI, Y
KIHARA, H
OHNO, M
机构
[1] KYUSHU UNIV, FAC SCI, DEPT CHEM, BIOCHEM LAB, FUKUOKA 812, JAPAN
[2] TAKARA SHUZO CO, BIOTECHNOL RES LABS, SHIGA 52021, JAPAN
[3] UNIV TOKYO, INST MAT SCI, TOKYO 108, JAPAN
关键词
D O I
10.1073/pnas.90.13.5964
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Six Trimeresurus flavoviridis (Habu snake) venom gland phospholipase A2 (PLA2) isozyme genes were found to consist of four exons and three introns and to encode proteins of 138 amino acid residues, including the signal sequence of 16 amino acid residues. Comparison of the nucleotide sequences showed that the introns are much more homologous than the protein-coding regions of exons except for the signal peptide-coding region of the first exon. The numbers of nucleotide substitutions per site (K(N)) for introns are approximately one-fourth of the numbers of nucleotide substitutions per synonymous site (K(S)) for the protein-coding regions, indicating that the introns are unusually conserved. The absence of an apparent functional role for the introns suggests that the protein-coding regions, except for the signal peptide-coding domains, have evolved at greater substitution rates than introns. The fact that the numbers of nucleotide substitutions per nonsynonymous site (K(A)) are close to or larger than K(S) values for relevant pairs of genes revealed that Darwinian-type accelerated substitutions have occurred in the protein-coding regions or exons. This is compatible with the presence of PLA2 species with diverse physiological activities in the venom.
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收藏
页码:5964 / 5968
页数:5
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