PLANT-REGENERATION FROM CELL CULTURE-DERIVED PROTOPLASTS OF STATICE (LIMONIUM-PEREZII HUBBARD)

Protoplasts were isolated from leaf segment-derived suspension cultures of Limonium perezii Hubbard and cultured in Gellan Gum-solidified 1 2 Murashige and Skoog (MS) medium containing 1 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D), 1 mg/l naphthaleneacetic acid (NAA), 1 mg/l 6-benzylaminopurine (BA), 250 mg/l casein hydrolysate, 3% (w/v) sucrose and 0.5 M mannitol. The protoplasts started to divide within 2-3 days of culture and formed colonies (0.5-1 mm) after 2 months of culture. These colonies were then transferred to MS medium containing 1 mg/l 2,4-D, 3% sucrose and 0.2% (w/v) Gellan Gum for callus proliferation. The calli were then transferred for shoot regeneration to MS medium with or without 2 mg/l zeatin. These shoots were rooted on MS medium containing 0.5 mg/l indole-3-butyric acid (IBA), 3% sucrose and 0.2% Gellan Gum and transferred to pots. No abnormalities in leaf shape and growth habit were observed in these plants. © 1990.