CHARACTERIZATION OF ADULT HUMAN ASTROCYTES DERIVED FROM EXPLANT CULTURE

被引：30
作者：
ESTES, ML
RANSOHOFF, RM
MCMAHON, JT
JACOBS, BS
BARNA, BP
机构：
[1] CLEVELAND CLIN FDN, DEPT NEUROL, CLEVELAND, OH 44195 USA
[2] CLEVELAND CLIN FDN, DEPT IMMUNOPATHOL, CLEVELAND, OH 44195 USA
[3] CLEVELAND CLIN FDN, DEPT MOLEC BIOL, CLEVELAND, OH 44195 USA
[4] CLEVELAND CLIN FDN, MELLEN CTR MULTIPLE SCLEROSIS TREATMENT & RES, CLEVELAND, OH 44195 USA
来源：
JOURNAL OF NEUROSCIENCE RESEARCH | 1990年 / 27卷 / 04期
关键词：
CELL CULTURE; GLIAL FIBRILLARY ACIDIC PROTEIN; ULTRASTRUCTURE; PROLIFERATION;
D O I：
10.1002/jnr.490270430
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Four different human astrocytic cell lines established from either epilepsy surgical specimens or cerebral white matter obtained during thalamotomy for tremor in a patient with multiple sclerosis were characterized using morphologic analysis, ultrastructural attributes, growth characteristics, and immunocytochemical analysis. Immunocytochemical characterization of cultures indicated a mean of 84% of cells contained cytoplasmic glial fibrillary acidic protein (GFAP): to confirm that GFAP(+) cells also proliferated, bromo-deoxyuridine (BrdU) uptake was measured in cell line. Our method of simplified explant culture allows establishment of astrocytic cell lines from a variety of pathologic substrates using limited amounts of human material.
引用
收藏
页码：697 / 705
页数：9
相关论文
共 29 条
[1]   IMMUNOFLUORESCENCE STAINING OF ASTROCYTES INVITRO USING ANTISERUM TO GLIAL FIBRILLARY ACIDIC PROTEIN [J].
ANTANITUS, DS ;
CHOI, BH ;
LAPHAM, LW .
BRAIN RESEARCH, 1975, 89 (02) :363-367
[2]  
BARNA B, 1989, ANN NEUROL, V26, P149
[3]   INTERFERON-BETA IMPAIRS INDUCTION OF HLA-DR ANTIGEN EXPRESSION IN CULTURED ADULT HUMAN ASTROCYTES [J].
BARNA, BP ;
CHOU, SM ;
JACOBS, B ;
YENLIEBERMAN, B ;
RANSOHOFF, RM .
JOURNAL OF NEUROIMMUNOLOGY, 1989, 23 (01) :45-53
[4]   LOCALIZATION OF VIMENTIN, THE NONSPECIFIC INTERMEDIATE FILAMENT PROTEIN, IN EMBRYONAL GLIA AND IN EARLY DIFFERENTIATING NEURONS - INVIVO AND INVITRO IMMUNOFLUORESCENCE STUDY OF THE RAT EMBRYO WITH VIMENTIN AND NEUROFILAMENT ANTISERA [J].
BIGNAMI, A ;
RAJU, T ;
DAHL, D .
DEVELOPMENTAL BIOLOGY, 1982, 91 (02) :286-295
[5]   GLIAL FIBRILLARY ACIDIC PROTEIN IN PRIMARY ASTROGLIAL CELL-CULTURES DERIVED FROM NEWBORN RAT-BRAIN [J].
BOCK, E ;
MOLLER, M ;
NISSEN, C ;
SENSENBRENNER, M .
FEBS LETTERS, 1977, 83 (02) :207-211
[6]  
BOOHER J, 1972, Neurobiology (Copenhagen), V2, P97
[7]  
BOVOLENTA P, 1984, DEV BIOL, V102, P248, DOI 10.1016/0012-1606(84)90189-1
[8]   THE VIMENTIN-GFA PROTEIN TRANSITION IN RAT NEUROGLIA CYTOSKELETON OCCURS AT THE TIME OF MYELINATION [J].
DAHL, D .
JOURNAL OF NEUROSCIENCE RESEARCH, 1981, 6 (06) :741-748
[9]   ACIDIC PROTEIN ISOLATED FROM FIBROUS ASTROCYTES [J].
ENG, LF ;
VANDENHA.JJ ;
BIGNAMI, A ;
GERSTL, B .
BRAIN RESEARCH, 1971, 28 (02) :351-&
[10]   HUMAN-BRAIN IN TISSUE-CULTURE .5. IDENTIFICATION OF GLIAL CELLS BY IMMUNOFLUORESCENCE [J].
GILDEN, DH ;
WROBLEWSKA, Z ;
ENG, LF ;
RORKE, LB .
JOURNAL OF THE NEUROLOGICAL SCIENCES, 1976, 29 (2-4) :177-184
123