THE USE OF FAB' FRAGMENTS IN A SCREENING METHOD FOR THE DETECTION OF HUMAN ANTITUMOR MONOCLONAL-ANTIBODIES

被引：1

作者：

FOULDS, S ^{[1
]}

EREMIN, O ^{[1
]}

机构：

[1] UNIV ABERDEEN,SCH MED,DEPT SURG,ABERDEEN AB9 2ZD,SCOTLAND

来源：

HYBRIDOMA | 1990年 / 9卷 / 01期

关键词：

D O I：

10.1089/hyb.1990.9.91

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of immunoperoxidase staining methods to detect human monoclonal antibodies that react with frozen tissue sections of human tumors is limited due to endogenous immunoglobulin present in the sections. The endogenous immunoglobulin produces a significant background staining which makes the detection of a specific reaction difficult and unreliable. A method of incubating the sections with papain fragments (Fab') of goat anti-human immunoglobulin was evaluated. It was demonstrated that Fab' fragments of goat anti-human polyvalent immunoglobulin can block endogenous immunoglobulin background staining after prolonged washing of tissue sections (20h) and thus facilitating the screening of monoclonal antibodies against frozen sections of tumor tissue.

引用

页码：91 / 96

页数：6