CA2+-BRIDGING MECHANISM AND PHOSPHOLIPID HEAD GROUP RECOGNITION IN THE MEMBRANE-BINDING PROTEIN ANNEXIN-V

被引：284
作者：
SWAIRJO, MA
CONCHA, NO
KAETZEL, MA
DEDMAN, JR
SEATON, BA
机构：
[1] BOSTON UNIV,SCH MED,DEPT PHYSIOL,STRUCT BIOL GRP,BOSTON,MA 02118
[2] UNIV CINCINNATI,COLL MED,DEPT MOLEC & CELLULAR PHYSIOL,CINCINNATI,OH 45267
来源：
NATURE STRUCTURAL BIOLOGY | 1995年 / 2卷 / 11期
关键词：
D O I：
10.1038/nsb1195-968
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Structural evidence is presented for a 'Ca-2'-bridging' mechanism, proposed for Ca2+-binding interfacial membrane proteins such as annexins, protein kinase C, and certain coagulation proteins, crystal structures of Ca2+-annexin V complexes with phospholipid polar heads provide molecular details of 'Ca2+-bridges' as key features in the membrane attachment exhibited by these proteins, Distinct binding sites for phospholipid head groups are observed, including a novel, double-Ca2+ recognition site for phosphoserine that may serve as a phosphatidylserine receptor site in vivo.
引用
收藏
页码：968 / 974
页数：7
相关论文
共 36 条
[1]  
ANDREE HAM, 1990, J BIOL CHEM, V265, P4923
[2]   STRUCTURE OF CHICKEN ANNEXIN-V AT 2.25-ANGSTROM RESOLUTION [J].
BEWLEY, MC ;
BOUSTEAD, CM ;
WALKER, JH ;
WALLER, DA .
BIOCHEMISTRY, 1993, 32 (15) :3923-3929
[3]   CHARACTERIZATION OF CA-2+-DEPENDENT PHOSPHOLIPID BINDING, VESICLE AGGREGATION AND MEMBRANE-FUSION BY ANNEXINS [J].
BLACKWOOD, RA ;
ERNST, JD .
BIOCHEMICAL JOURNAL, 1990, 266 (01) :195-200
[4]  
BRUNGER A, 1992, X PLOR 3 0 MANUAL
[5]   ANNEXIN-V FORMS CALCIUM-DEPENDENT TRIMERIC UNITS ON PHOSPHOLIPID-VESICLES [J].
CONCHA, NO ;
HEAD, JF ;
KAETZEL, MA ;
DEDMAN, JR ;
SEATON, BA .
FEBS LETTERS, 1992, 314 (02) :159-162
[6]   RAT ANNEXIN-V CRYSTAL-STRUCTURE - CA2+-INDUCED CONFORMATIONAL-CHANGES [J].
CONCHA, NO ;
HEAD, JF ;
KAETZEL, MA ;
DEDMAN, JR ;
SEATON, BA .
SCIENCE, 1993, 261 (5126) :1321-1324
[7]   CALCIUM AND MEMBRANE-BINDING PROPERTIES OF MONOMERIC AND MULTIMERIC ANNEXIN-II [J].
EVANS, TC ;
NELSESTUEN, GL .
BIOCHEMISTRY, 1994, 33 (45) :13231-13238
[8]  
GROCHULSKI P, 1993, J BIOL CHEM, V268, P12843
[9]   CRYSTAL AND MOLECULAR-STRUCTURE OF HUMAN ANNEXIN-V AFTER REFINEMENT - IMPLICATIONS FOR STRUCTURE, MEMBRANE-BINDING AND ION CHANNEL FORMATION OF THE ANNEXIN FAMILY OF PROTEINS [J].
HUBER, R ;
BERENDES, R ;
BURGER, A ;
SCHNEIDER, M ;
KARSHIKOV, A ;
LUECKE, H ;
ROMISCH, J ;
PAQUES, E .
JOURNAL OF MOLECULAR BIOLOGY, 1992, 223 (03) :683-704
[10]   THE CRYSTAL AND MOLECULAR-STRUCTURE OF HUMAN ANNEXIN-V, AN ANTICOAGULANT PROTEIN THAT BINDS TO CALCIUM AND MEMBRANES [J].
HUBER, R ;
ROMISCH, J ;
PAQUES, EP .
EMBO JOURNAL, 1990, 9 (12) :3867-3874
1234