ADSORPTION OF AEROSIL ON ERYTHROCYTE SURFACE BY FLOW-CYTOMETRY MEASUREMENTS

被引：10
|
作者：
GERASHCHENKO, BI [1 ]
GERASHCHENKO, II [1 ]
BOGOMAZ, VI [1 ]
PANTAZIS, CG [1 ]
机构：
[1] KIEV SURFACE CHEM INST,KIEV,UKRAINE
来源：
CYTOMETRY | 1994年 / 15卷 / 01期
关键词：
AEROSIL PARTICLES; ERYTHROCYTES; ADSORPTION; CELL-AEROSIL CONJUGATES; HEMOLYSIS; FLOW CYTOMETRY;
D O I：
10.1002/cyto.990150113
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Light scattering properties of the erythrocytes in less than 0.01% aerosil suspension were measured. It was shown that light scattering properties of intact cells and cells associated with silica (aerosil) are different. It was also established that the aerosil particles are characterized by more intensive autofluorescence in the channel of green fluorescence than the erythrocytes. We propose that this observation allows a facile means of studying the interaction of purified silica and cell membranes. Hemolysis, as a result of interaction between the aerosil particles and the erythrocytes, was confirmed by photometric measurement of hemoglobin in the supernatant. (C) 1994 Wiley-Liss, Inc.
引用
收藏
页码：80 / 83
页数：4
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