HYDROXYL RADICAL FORMATION BY UV-IRRADIATED EPIDERMAL-CELLS

被引：51

作者：

TAIRA, J ^{[1
]}

MIMURA, K ^{[1
]}

YONEYA, T ^{[1
]}

HAGI, A ^{[1
]}

MURAKAMI, A ^{[1
]}

MAKINO, K ^{[1
]}

机构：

[1] KYOTO INST TECHNOL,DEPT POLYMER SCI & ENGN,SAKYO KU,KYOTO 606,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1992年 / 111卷 / 06期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a123820

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To elucidate the mechanism of sunlight-induced skin damage, guinea pigs were exposed to UV light (280-320 nm, UV B, 4J/cm2) and a homogenate of the epidermis was examined by means of the thiobarbituric acid (TBA) test. Three hours after the exposure, TBA-malondialdehyde adducts had increased while glutathione reductase activity had decreased, indicating lipid peroxidation. To detect the initial species, spin trapping with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) was applied to a suspension of illuminated epidermal cells (0.5 J/cm2). An ESR signal obtained only with irradiation comprised a 1 : 2 : 2 : 1 quartet [a(N)=a(beta-H)=1.49mT] attributable to a spin adduct of hydroxyl radicals. These results suggest that sunlight exposure of skin may lead to hydroxyl radical generation and simultaneous lipid peroxidation.

引用

页码：693 / 695

页数：3

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