AN ALTERNATIVE EXPEDITIOUS ANALYSIS OF PHOSPHOLIPID-COMPOSITION IN HUMAN BLOOD-PLASMA BY P-31 NMR-SPECTROSCOPY

被引：50
作者：
BRADAMANTE, S
BARCHIESI, E
BARENGHI, L
ZOPPI, F
机构：
[1] FDN RIVETTI,MILAN,ITALY
[2] OSPED NIGUARDA CA GRANDA,BIOCHIM CLIN & EMATOL LAB,MILAN,ITALY
[3] UNIV MILAN,DIPARTIMENTO CHIM ORGAN & IND,I-20122 MILAN,ITALY
来源：
ANALYTICAL BIOCHEMISTRY | 1990年 / 185卷 / 02期
关键词：
D O I：
10.1016/0003-2697(90)90297-M
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
31P nuclear magnetic resonance spectroscopy has been applied to quantitate phospholipids in human blood plasma and in separate lipoprotein fractions. The addition of suitable detergents to samples produces an excellent chemical shift dispersion and allows the identification and integration of the peaks of the most important phospholipids. Results are in agreement with those obtained with enzymatic colorimetric and TLC methods: our method is characterized by good accuracy and reproducibility. © 1990.
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页码：299 / 303
页数：5
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