THE ESCHERICHIA-COLI CELL-SURFACE SPECIFICALLY PREVENTS THE INITIATION OF DNA-REPLICATION AT ORIC ON HEMIMETHYLATED DNA TEMPLATES

被引：73
作者：
LANDOULSI, A
MALKI, A
KERN, R
KOHIYAMA, M
HUGHES, P
机构：
[1] Institut Jacques Monod Tour 43 Université Paris VII, 75251 Paris Cedex 05
来源：
CELL | 1990年 / 63卷 / 05期
关键词：
D O I：
10.1016/0092-8674(90)90508-C
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A particular outer membrane fraction previously defined as possessing specific affinity for the hemimethylated form of the origin of replication of the E. coli chromosome (oriC) is shown to inhibit the initiation of DNA synthesis at this site on hemimethylated DNA templates in vitro. The replication of fully methylated or unmethylated DNA templates is not affected. Also, no inhibition is observed if initiation takes place at random sites on the hemimethylated template. The key inactivation step appears to be membrane inhibition of DnaA initiator protein binding to oriC. Remethylation of the membrane-bound hemimethylated DNA results in reactivation. Our results demonstrate direct involvement of the membrane in the control of DNA replication. We propose that association/dissociation of the origin from the cell membrane is one of the control elements governing interintiation times in E. coli. © 1990.
引用
收藏
页码：1053 / 1060
页数：8
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