LOCATION OF DISULFIDE BONDS IN ANTITHROMBIN-III

被引：17
作者：
ZHOU, ZR [1 ]
SMITH, DL [1 ]
机构：
[1] PURDUE UNIV,DEPT MED CHEM & PHARMACOGNOSY,W LAFAYETTE,IN 47907
来源：
BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY | 1990年 / 19卷 / 12期
关键词：
D O I：
10.1002/bms.1200191206
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
Proteolytic digests of human antithrombin III (ATIII) have been analyzed by a combination of reversed‐phase high‐performance liquid chromatography and fast atom bombardment (FAB) mass spectrometry for disulfidecontaining peptides which are diagnostic for disulfide linkages in ATIII. These results indicate that disulfide bonds join Cys 8 and Cys 128, Cys 21 and Cys 95, and Cys 247 and Cys 430. In addition, these results demonstrate that systematic searches of the amino acid sequence of a large protein for segments with molecular weights matching FAB mass spectral information is a viable method for identifying peptides independent of the specificity of the enzyme used to fragment the protein. Copyright © 1990 John Wiley & Sons, Ltd.
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页码：782 / 786
页数：5
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