In vitro Evaluation of Antioxidant Potential of Isolated Compounds and Various Extracts of Peel of Punica granatum L.

被引：11

作者：

Jacob, Janani ^{[1
,3
]}

Lakshmanapermalsamy, P. ^{[1
,2
]}

Illuri, Ramanaiah ^{[3
]}

Bhosle, Damaji ^{[3
]}

Sangli, Gopala Krishna ^{[3
]}

Mundkinajeddu, Deepak ^{[3
]}

机构：

[1] Karpagam Univ, Karpagam Acad Higher Educ, Coimbatore, Tamil Nadu, India

[2] Bharathiar Univ, Dept Environm Sci, Coimbatore, Tamil Nadu, India

[3] Nat Remedies Pvt Ltd, R&D Ctr, Bengaluru, Karnataka, India

来源：

PHARMACOGNOSY RESEARCH | 2018年 / 10卷 / 01期

关键词：

2,3-(S)-hexahydroxydiphenoyl-D-glucose; antioxidant; nuclear magnetic resonance; Punica granatum;

D O I：

10.4103/pr.pr_36_17

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Background: Punica granatum L. (Lythraceae) peel has been proven to exhibit widespread pharmacological application against multitude of diseases due to the presence of bioactive principles. Objective: The objective is to isolate the bioactive compounds from the pericarp of P. granatum and to evaluate the antioxidant activity of various extracts. Materials and Methods: Dried peel of P. granatum was extracted with aqueous acetone and chromatographed on Diaion HP-20. Enriched fractions were rechromatographed on Sephadex LH-20 and purified on preparative high-performance liquid chromatography to identify individual compounds. The dried peel was extracted with different solvents to evaluate the antioxidant activity of the extracts. Results: On the chemical investigation, three compounds were isolated and characterized as punicalagin, 2,3-(S)-hexahydroxydiphenoyl-D-glucose, and punicalin, using various spectroscopic techniques. Conclusion: Results indicate that the isolated compounds have possessed antioxidant activity, and aqueous, methanol, and aqueous acetone extract showed significant scavenging of 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) radicals.

引用

页码：44 / 48

页数：5

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