CLONING, SEQUENCE-ANALYSIS AND EXPRESSION OF THE GROUP-A STREPTOCOCCAL GUAB GENE ENCODING INOSINE MONOPHOSPHATE DEHYDROGENASE

被引：10

作者：

ASHBAUGH, CD

WESSELS, MR

机构：

[1] BRIGHAM & WOMENS HOSP, CHANNING LAB, BOSTON, MA 02115 USA

[2] HARVARD UNIV, BETH ISRAEL HOSP, SCH MED, DIV INFECT DIS, BOSTON, MA 02115 USA

来源：

GENE | 1995年 / 165卷 / 01期

关键词：

D O I：

10.1016/0378-1119(95)00422-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Inosine monophosphate dehydrogenase (IMPDH) is an essential enzyme in the biosynthesis of purines. We cloned a group A streptococcal (GAS) DNA fragment containing an open reading frame similar to other bacterial guaB genes encoding IMPDH. The GAS guaB consists of 1479 nucleotides encoding a protein of 493 amino acids. Expression of the GAS guaB in an Escherichia coli guaB mutant restored IMPDH activity, confirming the function of the gene product and demonstrating that the GAS enzyme is active in a heterologous bacterial host. Restriction mapping and Southern hybridization analysis of GAS chromosomal DNA localized guaB to a site approximately 5 kb from the hasA and hasB genes which encode enzymes necessary for hyaluronic acid capsule synthesis.

引用

页码：57 / 60

页数：4

共 15 条

[11]

SHIMURA K, 1983, J BIOCHEM, V94, P1595

[12] NUCLEOTIDE-SEQUENCE OF THE GUAB LOCUS ENCODING IMP DEHYDROGENASE OF ESCHERICHIA-COLI-K12 [J].