TRANSCRIPTIONAL ACTIVATION OF THE IL-6 RESPONSE ELEMENT IN THE JUNB PROMOTER IS MEDIATED BY MULTIPLE STAT FAMILY PROTEINS

被引：58

作者：

FUJITANI, Y ^{[1
]}

NAKAJIMA, K ^{[1
]}

KOJIMA, H ^{[1
]}

NAKAE, K ^{[1
]}

TAKEDA, T ^{[1
]}

HIRANO, T ^{[1
]}

机构：

[1] OSAKA UNIV, SCH MED, BIOMED RES CTR, DIV MOLEC ONCOL, SUITA, OSAKA 565, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 202卷 / 02期

关键词：

D O I：

10.1006/bbrc.1994.2053

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

IL-6 signals activate an IL-6 response element in the junB promoter, JRE-IL6, in a Ras-independent manner. IL-6 rapidly induced a DNA-binding activity to the Ets binding site of JRE-IL6 (JEBS), one of necessary DNA motifs of the IL-6 response element. The IL-6-induced JEBS-binding activity was indistinguishable from those to acute phase response element (APRE) in both kinetics of induction and its DNA-binding specificity. Purified APRE binding factors (APRFs) from IL-6-stimulated rat liver were found to be composed of multiple Stat3-related proteins and Stat1. Moreover the purified APRFs specifically made a complex with JRE-IL6 with the same mobility as that observed in the crude extracts. These results indicate that an immediate early signal of IL-6 leading to activation of JRE-IL6 is mediated by STAT family transcription factors. (C) 1994 Academic Press, Inc.

引用

页码：1181 / 1187

页数：7

共 27 条

[1] MOLECULAR-CLONING OF APRF, A NOVEL IFN-STIMULATED GENE FACTOR-3 P91-RELATED TRANSCRIPTION FACTOR INVOLVED IN THE GP130-MEDIATED SIGNALING PATHWAY [J].

AKIRA, S ;

NISHIO, Y ;

INOUE, M ;

WANG, XJ ;

WEI, S ;

MATSUSAKA, T ;

YOSHIDA, K ;

SUDO, T ;

NARUTO, M ;

KISHIMOTO, T .

CELL, 1994, 77 (01) :63-71

[2] ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI [J].

DIGNAM, JD ;

LEBOVITZ, RM ;

ROEDER, RG .

NUCLEIC ACIDS RESEARCH, 1983, 11 (05) :1475-1489

[3] A TRANSCRIPTION FACTOR WITH SH2 AND SH3 DOMAINS IS DIRECTLY ACTIVATED BY AN INTERFERON-ALPHA-INDUCED CYTOPLASMIC PROTEIN TYROSINE KINASE(S) [J].

FU, XY .

CELL, 1992, 70 (02) :323-335

[4] TRANSCRIPTION FACTOR P91 INTERACTS WITH THE EPIDERMAL GROWTH-FACTOR RECEPTOR AND MEDIATES ACTIVATION OF THE C-FOS GENE PROMOTER [J].

FU, XY ;

ZHANG, JJ .

CELL, 1993, 74 (06) :1135-1145

[5] ACUTE-PHASE REACTION INDUCES A SPECIFIC COMPLEX BETWEEN HEPATIC NUCLEAR PROTEINS AND THE INTERLEUKIN-6 RESPONSE ELEMENT OF THE RAT ALPHA-2-MACROGLOBULIN GENE [J].

HATTORI, M ;

ABRAHAM, LJ ;

NORTHEMANN, W ;

FEY, GH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (06) :2364-2368

[6] MOLECULAR-CLONING AND EXPRESSION OF AN IL-6 SIGNAL TRANSDUCER, GP130 [J].

HIBI, M ;

MURAKAMI, M ;

SAITO, M ;

HIRANO, T ;

TAGA, T ;

KISHIMOTO, T .

CELL, 1990, 63 (06) :1149-1157

[7]

HIRANO T, 1992, CHEM IMMUNOL, V51, P153

[8]

KADONAGA JT, 1991, METHOD ENZYMOL, V208, P10

[9] INDUCTION OF THE LY-6A/E GENE BY INTERFERON-ALPHA/BETA AND INTERFERON-GAMMA REQUIRES A DNA ELEMENT TO WHICH A TYROSINE-PHOSPHORYLATED 91-KDA PROTEIN BINDS [J].

KHAN, KD ;

SHUAI, K ;

LINDWALL, G ;

MAHER, SE ;

DARNELL, JE ;

BOTHWELL, ALM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (14) :6806-6810

[10] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

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