A RAPID METHOD FOR MEASURING SERUM AMYLOID-A PROTEIN BY LATEX AGGLUTINATION NEPHELOMETRIC IMMUNOASSAY

被引：39

作者：

YAMADA, T ^{[1
]}

NOMATA, Y ^{[1
]}

SUGITA, O ^{[1
]}

OKADA, M ^{[1
]}

机构：

[1] EIKEN CHEM CO LTD,TOKYO RES LAB,KATSUSHIKA KU,TOKYO 124,JAPAN

来源：

ANNALS OF CLINICAL BIOCHEMISTRY | 1993年 / 30卷

关键词：

KINETIC ASSAY; ACUTE PHASE REACTANT; HIGH DENSITY LIPOPROTEIN;

D O I：

10.1177/000456329303000112

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Serum amyloid A protein (SAA) is a sensitive acute phase reactant. We developed a method for the rapid measurement of human SAA in serum by kinetic nephelometry of anti-SAA antibody-coated latex agglutination. Measurement takes less than 6 min using an automated analyser. Standardization of the assay employs SAA-enriched high density lipoprotein as the primary standard. The values determined by our new method and by conventional enzymeimmunoassay showed good agreement (r = 0.988). The normal range was 0.17 - 10.0 mg/L [mean (SD)]. This rapid method should prove useful in clinical laboratories.

引用

页码：72 / 76

页数：5

共 3 条

[1] CHARACTERIZATION OF CONSTITUTIVE HUMAN SERUM AMYLOID-A PROTEIN (SAA(4)) AS AN APOLIPOPROTEIN
DEBEER, MC
YUAN, T
KINDY, MS
ASZTALOS, BF
ROHEIM, PS
DEBEER, FC
JOURNAL OF LIPID RESEARCH, 1995, 36 (03) : 526 - 534
[2] SERUM AMYLOID-A PROTEIN IN PATIENTS WITH NON-INSULIN-DEPENDENT DIABETES-MELLITUS
KUMON, Y
SUEHIRO, T
ITAHARA, T
IKEDA, Y
HASHIMOTO, K
CLINICAL BIOCHEMISTRY, 1994, 27 (06) : 469 - 473
[3] QUANTIFICATION AND MAPPING OF ANTIGENIC DETERMINANTS OF SERUM AMYLOID-A (SAA) PROTEIN UTILIZING SEQUENCE-SPECIFIC IMMUNOGLOBULINS AND EU3+ AS A SPECIFIC PROBE FOR TIME-RESOLVED FLUOROMETRIC IMMUNOASSAY
MALLE, E
MUNSCHER, G
MULLER, T
VERMEER, H
IBOVNIK, A
JOURNAL OF IMMUNOLOGICAL METHODS, 1995, 182 (01) : 131 - 144

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