CROMAKALIM, A VASODILATOR, DIFFERENTIALLY INHIBITS CA-2+ CURRENTS IN NG108-15 NEUROBLASTOMA X GLIOMA HYBRID-CELLS

被引：9

作者：

ITO, Y ^{[1
]}

MIYAMORI, I ^{[1
]}

MATSUBARA, T ^{[1
]}

TAKEDA, R ^{[1
]}

HIGASHIDA, H ^{[1
]}

机构：

[1] KANAZAWA UNIV,SCH MED,DEPT INTERNAL MED,KANAZAWA,ISHIKAWA 920,JAPAN

来源：

FEBS LETTERS | 1990年 / 262卷 / 02期

关键词：

(NG108-15 cell); Ca[!sup]2+[!/sup] inward current; Cromakalim; L-type; Patch clamp; T-type;

D O I：

10.1016/0014-5793(90)80217-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Extracellular perfusion with the antihypertensive agent cromakalim produced an inhibition of 22-66% in the low-threshold transient Ca2+ (T-like) current in NG108-15 hybrid cells. Cromakalim suppressed the high-threshold and long-lasting Ba2+ current (L-like Ca2+ current) by 29-73%, but had almost no effect on the high-threshold and inactivating Ba2+ current (N-like Ca2+ current). IC50 for T-like and L-like currents was the same at about 100 μM. The inhibitory effect developed relatively fast and was reversible. These results indicate that cromakalim can selectively inhibit the activity of inward Ca2+ currents. © 1990.

引用

页码：313 / 316

页数：4

共 13 条

[1] VOLTAGE-ACTIVATED AND CALCIUM-ACTIVATED POTASSIUM CURRENTS IN MOUSE NEUROBLASTOMAXRAT GLIOMA HYBRID-CELLS [J].

BROWN, DA ;

HIGASHIDA, H .

JOURNAL OF PHYSIOLOGY-LONDON, 1988, 397 :149-165

[2] THE PHARMACOLOGY OF POTASSIUM CHANNELS AND THEIR THERAPEUTIC POTENTIAL [J].

COOK, NS .

TRENDS IN PHARMACOLOGICAL SCIENCES, 1988, 9 (01) :21-28

[3] NORADRENALINE-INDUCED INHIBITION OF VOLTAGE-SENSITIVE CALCIUM CURRENTS IN NG108-15 HYBRID-CELLS [J].

DOCHERTY, RJ ;

MCFADZEAN, I .

EUROPEAN JOURNAL OF NEUROSCIENCE, 1989, 1 (02) :132-140

[4] POTASSIUM CHANNEL OPENERS ACT THROUGH AN ACTIVATION OF ATP-SENSITIVE K+ CHANNELS IN GUINEA-PIG CARDIAC MYOCYTES [J].

ESCANDE, D ;

THURINGER, D ;

LEGUERN, S ;

COURTEIX, J ;

LAVILLE, M ;

CAVERO, I .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1989, 414 (06) :669-675

[5] KINETIC AND PHARMACOLOGICAL PROPERTIES DISTINGUISHING 3 TYPES OF CALCIUM CURRENTS IN CHICK SENSORY NEURONS [J].

FOX, AP ;

NOWYCKY, MC ;

TSIEN, RW .

JOURNAL OF PHYSIOLOGY-LONDON, 1987, 394 :149-172

[6] COMPARISON OF THE EFFECTS OF BRL-34915 AND VERAPAMIL ON ELECTRICAL AND MECHANICAL-ACTIVITY IN RAT PORTAL-VEIN [J].

HAMILTON, TC ;

WEIR, SW ;

WESTON, AH .

BRITISH JOURNAL OF PHARMACOLOGY, 1986, 88 (01) :103-111

[7]

MCFADZEAN I, 1989, ABSTR SOC NEUROSCI, V15, P16

[8]

NARAHASHI T, 1987, J PHYSIOL-LONDON, V383, P231

[9] MODULATION OF SYNAPSE FORMATION BY CYCLIC ADENOSINE-MONOPHOSPHATE [J].

NIRENBERG, M ;

WILSON, S ;

HIGASHIDA, H ;

ROTTER, A ;

KRUEGER, K ;

BUSIS, N ;

RAY, R ;

KENIMER, JG ;

ADLER, M .

SCIENCE, 1983, 222 (4625) :794-799

[10]

OKADE K, IN PRESS BR J PHARM

← 1 2 →