STUDIES ON THE ACTIVITY OF PHOSPHOLIPASE-D WITH ALKYL PHOSPHORYLCHOLINES

被引：5

作者：

OHNO, Y ^{[1
]}

OKAZAKI, M ^{[1
]}

HARA, I ^{[1
]}

机构：

[1] TOKYO MED & DENT UNIV, DEPT GEN EDUC, ICHIKAWA, CHIBA 272, JAPAN

来源：

BIOCHEMISTRY AND CELL BIOLOGY | 1990年 / 68卷 / 01期

关键词：

D O I：

10.1139/o90-052

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The activity of phospholipase D from the Savoy cabbage on alkyl phosphorylcholines containing alkyl groups of C14 to C22 was examined. Apparent K(m) and maximal velocity values in the presence of diethyl ether were 3.6-8.3 mM and 0.14-0.29 μmol/(min.mg protein), respectively. The maximal velocities for alkyl phosphorylcholine homologues were lower than the value for pig phosphatidylcholines, but higher than those for lysophosphatidylcholines and synthetic phosphatidylcholine with saturated acyl chains. Results suggest that solubilities of the substrates in the organic solvent play an important role in activity observed with phospholipase D.

引用

页码：376 / 379

页数：4

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