PLASMODIUM-FALCIPARUM - DETECTION OF P-GLYCOPROTEIN IN CHLOROQUINE-SUSCEPTIBLE AND CHLOROQUINE-RESISTANT CLONES AND ISOLATES

被引：19
作者：
CREMER, G
BASCO, LK
LEBRAS, J
CAMUS, D
SLOMIANNY, C
机构：
[1] UNIV LYON 1,HOP BICHAT,PARASITOL LAB,F-75877 PARIS,FRANCE
[2] INSERM,U42,F-59651 VILLENEUVE DASCQ,FRANCE
来源：
EXPERIMENTAL PARASITOLOGY | 1995年 / 81卷 / 01期
关键词：
D O I：
10.1006/expr.1995.1086
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Recent studies have suggested the potential involvement of multidrug resistance (mdr) genes in resistance to quinoline-containing compounds in Plasmodium falciparum parasites. Three different antibodies were used to detect the malarial mdr 1 protein product by indirect immunofluorescence and/or immunoblot in fresh clinical isolates and clones of P. falciparum from different geographic origins. A 160-kDa protein was detected in all five parasite clones by immunoblot and around the food vacuole by IFA, regardless of the level of sensitivity to chloroquine and the modulation by desipramine of chloroquine accumulation. Our results suggest that chloroquine resistance is not correlated with the presence of the Pfmdr1 product. (C) 1995 Academic Press. Inc.
引用
收藏
页码：1 / 8
页数：8
相关论文
共 30 条
  • [1] SELECTION FOR HIGH-LEVEL CHLOROQUINE RESISTANCE RESULTS IN DEAMPLIFICATION OF THE PFMDR1 GENE AND INCREASED SENSITIVITY TO MEFLOQUINE IN PLASMODIUM-FALCIPARUM
    BARNES, DA
    FOOTE, SJ
    GALATIS, D
    KEMP, DJ
    COWMAN, AF
    [J]. EMBO JOURNAL, 1992, 11 (08) : 3067 - 3075
  • [2] IN-VITRO ACTIVITY OF ARTEMISININ DERIVATIVES AGAINST AFRICAN ISOLATES AND CLONES OF PLASMODIUM-FALCIPARUM
    BASCO, LK
    LEBRAS, J
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1993, 49 (03) : 301 - 307
  • [3] PLASMODIUM-FALCIPARUM - INVITRO DRUG-INTERACTION BETWEEN CHLOROQUINE AND ENANTIOMERS OF AMLODIPINE
    BASCO, LK
    LEBRAS, J
    [J]. EXPERIMENTAL PARASITOLOGY, 1991, 72 (03) : 262 - 270
  • [4] REVERSAL OF CHLOROQUINE RESISTANCE IN MALARIA PARASITE PLASMODIUM-FALCIPARUM BY DESIPRAMINE
    BITONTI, AJ
    SJOERDSMA, A
    MCCANN, PP
    KYLE, DE
    ODUOLA, AMJ
    ROSSAN, RN
    MILHOUS, WK
    DAVIDSON, DE
    [J]. SCIENCE, 1988, 242 (4883) : 1301 - 1303
  • [5] INTERNAL DUPLICATION AND HOMOLOGY WITH BACTERIAL TRANSPORT PROTEINS IN THE MDR1 (P-GLYCOPROTEIN) GENE FROM MULTIDRUG-RESISTANT HUMAN-CELLS
    CHEN, CJ
    CHIN, JE
    UEDA, K
    CLARK, DP
    PASTAN, I
    GOTTESMAN, MM
    RONINSON, IB
    [J]. CELL, 1986, 47 (03) : 381 - 389
  • [6] THE P-GLYCOPROTEIN HOMOLOGS OF PLASMODIUM-FALCIPARUM - ARE THEY INVOLVED IN CHLOROQUINE RESISTANCE
    COWMAN, AF
    [J]. PARASITOLOGY TODAY, 1991, 7 (04): : 70 - 76
  • [7] A P-GLYCOPROTEIN HOMOLOG OF PLASMODIUM-FALCIPARUM IS LOCALIZED ON THE DIGESTIVE VACUOLE
    COWMAN, AF
    KARCZ, S
    GALATIS, D
    CULVENOR, JG
    [J]. JOURNAL OF CELL BIOLOGY, 1991, 113 (05) : 1033 - 1042
  • [8] FOJO A, 1985, CANCER RES, V45, P3002
  • [9] SEVERAL ALLELES OF THE MULTIDRUG-RESISTANCE GENE ARE CLOSELY LINKED TO CHLOROQUINE RESISTANCE IN PLASMODIUM-FALCIPARUM
    FOOTE, SJ
    KYLE, DE
    MARTIN, RK
    ODUOLA, AMJ
    FORSYTH, K
    KEMP, DJ
    COWMAN, AF
    [J]. NATURE, 1990, 345 (6272) : 255 - 258
  • [10] AMPLIFICATION OF THE MULTIDRUG RESISTANCE GENE IN SOME CHLOROQUINE-RESISTANT ISOLATES OF P-FALCIPARUM
    FOOTE, SJ
    THOMPSON, JK
    COWMAN, AF
    KEMP, DJ
    [J]. CELL, 1989, 57 (06) : 921 - 930
123